AE-200100-1 Porcine Toxoplasmosis IgG Antibody kit, 96 tests Detect Ab(Serum)猪弓形虫抗体ELISA检测试剂盒

使用步骤：

Step 1. Pipet 100 ul of each pre-diluted positive serum, pre-diluted negative, control (containing only sample diluent), samples containing Toxoplasmosis antibodies (diluted as required) in duplicated wells. Mix gently and incubate at 37 ℃ for 30 min.

Step 2. Aspirate and wash the plate three times. Add 100ul of enzyme conjugate to all wells, mix gently and incubate at 37 ℃for 30 min.

Step 3. Aspirate and wash the plate three times. Add 50 ul of each substrate A and B solution to all wells, mix gently, and incubate in the dark at room temperature for 10 min.

Step 4. Pipet 50 ul of stop solution into each well and mix gently (blue color turns yellow). Measure OD at A630 nm. The samples are positive if the value is > than 0.4, skeptical if the value is between 0.2 and 0.4 and negative if < than 0.2.