AE-200140-1 猪霍乱病毒抗体Elisa检测试剂盒Hog Cholera Virus Antibody ELISA kit, 96 tests Control Disease

背景描述：

Hog Cholera is also called Classical swine fever (CSF). It is very contagious among pigs and wild boar. The virus responsible for this disease is called CSV. It is classified in the Group IV ((+) ssRNA), it is a lipid-enveloped pathogen which belongs to the genus Pest virus in the family of Flaviviridae. CSFV is very similar to a ruminant pestiviruses which cause Bovine Viral Diarrhoea (BVDV) and Border Disease

(BDV). Pigs and wild boars are the only hosts for CSV. The virus will live in the blood, tissues, secretions and excretions from the infected animal. It will be transmitted mostly by the oral route, conjunctiva, mucous membrane, skin abrasion, insemination and percutaneous blood transfer. Airborne transmission is rare but can occur through mechanically ventilated units to the next unit. Once the animal is infected the

incubation period is normally from 3 to 4 days but can range between 2 to 14 days. After four days to three weeks of the virus entered the animal’s system the symptoms will start with fever which will lead to loss of appetite, depression, withdrawal from other animals, reddened and draining eyes, vomiting, constipation or diarrhea, and coughing and difficulty in respiration. Other symptoms also include purple

hemorrhagic areas on the abdomen, nervous sign such as circling, incoordination, muscle tremors and even convulsions.

Genemed Synthesis’s Hog Cholera Virus Antibody Test kit is a highly sensitive indirect type assay for the detection of antibodies against

HCV in swine serum.

操作步骤：

Step 1. Pipet 100 ul of each pre-diluted positive serum, pre-diluted negative serum, control (containing only sample diluent) and, samples containing antibodies against HCV (diluted as required) in duplicates wells. Mix gently and incubate at 37 ℃ for 30min.

Step 2. Aspirate and wash the plate three times. Add 100ul of Anti-pig IgG-HRP Conjugate to all wells, mix gently and incubate at 37℃for 30 min.

Step 3. Aspirate and wash the plate three times. Add 50 ul of each substrate A and B solution to all wells, mix gently, and incubate in the dark at room temperature for 10 min.

Step 4. Pipet 50 ul of stop solution into each well and mix gently (blue color turns yellow). Measure OD at A630 nm. The samples are positive if the value is > than 0.42, skeptical if the value is between 0.38 and 0.42 and negative if < than 0.38.