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孔雀石绿ELISA试剂盒
2019-07-05
产品详情
- 产地 美国
- 产品名称孔雀石绿ELISA试剂盒
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- 简介
DE-100280 孔雀石绿ELISA试剂盒Malachite green ELISA kit (Fish, shrimp)
背景介绍：
孔雀石绿(MG)是一种水溶性很强的化学染料，在水产养殖业中用作杀菌剂和消毒剂。镁是潜在的，它可能会对食用水产养殖产品的人造成重大的健康风险。这导致禁止孔雀石绿处理用于食品生产的鱼和虾。孔雀石绿(MG)由于在水中暴露时易被鱼类吸附，并迅速代谢为孔雀石绿(LMG)，因此在渔业中得到了广泛的应用。然而，MG和LMG都是潜在的致癌物，致畸剂和诱变剂。
操作步骤：
Bring test kit to the room temperature (20-25 ℃) for at least 30 min, note that each reagent must be shaken evenly before use; put the required micro-well strips into plate frames. Resealed the unused microplate, stored at 2-8 ℃, not frozen.
1. Add 50 μL of the standard and sample in duplicate wells.
2. Add 50 μL of the antibody solution to each well, mix gently manually for 5-10 seconds. Cover the microplate and incubate at 25°C for 30 min.
3. Aspirate the plates and tap dry on absorbent paper. Wash the plate 5 times with 300 ul wash buffer (1X).
4. Add 100μL enzyme conjugate to each well. Mix gently manually for 5-10 second, cover the plate and incubate at 25 °C for 30 min. Aspirate the plates and tap dry on absorbent paper. Wash 5 times same as in step 3.
5. Add 50 μL of the substrate A solution followed by 50 μL of the B solution into each well. (alternatively it is possible to mix Soln A and B in 1:1 ratio in a clean tube, mix, and add 100 ul of the mix in a single step). Mix gently manually for 5-10 seconds. Cover the plate and incubate at 25 °C for 15-20 min in the dark (blue color develops in standards and samples).
6. Add 50 μL of the stop solution into each well. Mix gently manually for 5-10 seconds (blue color turns yellow). Read the plate at 450 nm to determine the OD value of every well. (Recommend to read the OD value at the dualwavelength 450/630nm) within 5-10 min.
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