鱼11-酮睾酮（KT）ELISA检测试剂盒
2019-07-05
产品详情
- 产地 美国
- 产品名称鱼11-酮睾酮（KT）ELISA检测试剂盒
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- 简介
DE-100740 鱼11-酮睾酮（KT）ELISA检测试剂盒11-keto Testosterone ELISA Kit (for fish), 96 tests
产品介绍：
鱼11-酮睾酮（KT）ELISA检测试剂盒是一种竞争性酶免疫分析试剂盒，用于定量分析样本中的11-酮睾酮。
使用步骤：
Step 1. Pipet 50ul each standards and samples containing KT (diluted as required). Pipet 100ul Antibody#1 to the wells and incubate it for 30min at 25 ℃.Aspirate and wash the plate 3 times.
Step 2. 150 ul of antibody#2-enzyme conjugate solution in duplicates into the wells. Seal the plate, mix gently and incubate at 25 ℃ for 30min.
Step 3. Aspirate and wash the plate five times.
Step 4. Add 100 ul of TMB substrate solution to all wells, mix gently, and incubate at 25 ℃ for 15 min in the dark for coloration.
Step 5. Pipet 100 ul of stop solution into each well and mix gently (blue color turns yellow). Measure OD at A450 nm.
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