猴免疫球蛋白a(iga)检测试剂盒
2019-08-07
产品详情
- 产地 美国
- 产品名称猴免疫球蛋白a(iga)检测试剂盒
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- 简介
EK-7010 猴免疫球蛋白A（IgA）检测试剂盒Monkey IgA ELISA Kit, 96 tests, Quantitative
产品介绍：
The Monkey IgA ELISA kit is based on the binding of monkey IgA in samples to two antibodies, one immobilized on the microtiter wells, and the other conjugated to horseradish peroxidase (HRP) enzyme. After a washing step, chromogenic
substrate is added and color is developed by the enzymatic reaction of HRP on the TMB substrate, which is directly proportional to the amount of IgA present in the sample. Stopping Solution is added to terminate the reaction, and absorbance at 450nm is then measured using an ELISA microtiter well reader. The concentration of IgA in samples and control is calculated from a curve of standards containing known concentrations of IgA.
ELISA的基础是抗原或抗体的固相化及抗原或抗体的酶标记。结合在固相载体表面的抗原或抗体仍保持其免疫学活性，酶标记的抗原或抗体既保留其免疫学活性，又保留酶的活性。在测定时，受检标本（测定其中的抗体或抗原）与固相载体表面的抗原或抗体起反应。用洗涤的方法使固相载体上形成的抗原抗体复合物与液体中的其他物质分开。再加入酶标记的抗原或抗体，也通过反应而结合在固相载体上。此时固相上的酶量与标本中受检物质的量呈一定的比例。加入酶反应的底物后，底物被酶催化成为有色产物，产物的量与标本中受检物质的量直接相关，故可根据呈色的深浅进行定性或定量分析。
其他猴免疫球蛋白试剂盒：
EK-7050 猴免疫球蛋白G（IgG）检测试剂盒Monkey IgG (total) ELISA Kit, 96 tests, Quantitative
EK-7060 猴免疫球蛋白M（IgM）检测试剂盒Monkey IgM ELISA Kit, 96 tests, Quantitative
EK-7070 猴免疫球蛋白E（IgE）检测试剂盒Monkey IgE ELISA Kit, 96 tests, Quantitative
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